The symptoms of immune hemolytic anemia include pale or yellowish skin color, tiredness or fatigue, fast heartbeat or heart rate, shortness of breath, enlarged spleen, and dark urine. The disease is diagnosed by performing several tests. The following indicate that the patient is suffering from the disease: Coombs’ test turning out positive direct or indirect; increased bilirubin levels; increased LDH level (LDH are enzymes that increase when the body is experiencing tissue damage); presence of hemoglobin in the urine; decreased serum haptoglobin level; increased reticulocyte count; and decreased hemoglobin and RBC count.
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Within the genitourinary and gastrointestinal tracts, commensal flora serve as biological barriers by competing with pathogenic bacteria for food and space and, in some cases, by changing the conditions in their environment, such as pH or available iron.  As a result of the symbiotic relationship between commensals and the immune system , the probability that pathogens will reach sufficient numbers to cause illness is reduced. However, since most antibiotics non-specifically target bacteria and do not affect fungi, oral antibiotics can lead to an "overgrowth" of fungi and cause conditions such as a vaginal candidiasis (a yeast infection).  There is good evidence that re-introduction of probiotic flora, such as pure cultures of the lactobacilli normally found in unpasteurized yogurt , helps restore a healthy balance of microbial populations in intestinal infections in children and encouraging preliminary data in studies on bacterial gastroenteritis , inflammatory bowel diseases , urinary tract infection and post-surgical infections .